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You are here: Home > Dose Estimator for Exposure: 3 Biodosimetry Tools > Biodosimetry Reference List

References: Biodosimetry, Lymphocyte Depletion Kinetics, Dicentric Chromosome Assay

Biodosimetry: value and limitations

Three Tables Comparing Biodosimetry Assays

  1. Cytogenetic aberration assays used for dose assessment (PDF - 64 KB),
    adapted from Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies, Table 1, page 3, (IAEA, PAHO, WHO, September 2011)
  2. Summary of advantages and disadvantages of existing and developing techniques for assessment of radiation exposure levels,
    adapted from High dose radiation effects and tissue injury, Report of the Independent Advisory Group on Ionising Radiation, Table 4.4, page 41 (Health Protections Agency, UK, March 2009) (PDF - 865 KB)
  3. Comparison of retrospective dosimetry assays (PDF - 97 KB),
    adpated from Ainsbury EA, Bakhanova E, Barquinero JF, et al., Review of retrospective dosimetry techniques for external ionising radiation exposure. Table 1, Radiat Prot Dosimetry. 2011;147(4):573-92. Epub 2010 Dec 23. [PubMed Citation]

Other references

  1. Flood AB, Nicolalde RJ, Demidenko E, Williams BB, Shapiro A, Wiley AL Jr, Swartz HM. A Framework for Comparative Evaluation of Dosimetric Methods to Triage a Large Population Following a Radiological Event. Radiat Meas. 2011 Sep 1;46(9):916-922. [PubMed Citation]
  2. Swartz HM, Flood AB, Gougelet RM, Rea ME, Nicolalde RJ, Williams BB. A critical assessment of biodosimetry methods for large-scale incidents. Health Phys. 2010 Feb;98(2):95-108. [PubMed Citation]
  3. Riecke A, Ruf CG, Meineke V. Assessment of radiation damage-the need for a multiparametric and integrative approach with the help of both clinical and biological dosimetry. Health Phys. 2010 Feb;98(2):160-7. [PubMed Citation]
  4. Vinnikov VA, Ainsbury EA, Maznyk NA, Lloyd DC, Rothkamm K. Limitations associated with analysis of cytogenetic data for biological dosimetry. Radiat Res. 2010 Oct;174(4):403-14. [PubMed Citation]
  5. Moulder JE. Post-irradiation approaches to treatment of radiation injuries in the context of radiological terrorism and radiation accidents: a review. Int J Radiat Biol. 2004 Jan;80(1):3-10. [PubMed Citation]

The importance of standardizing laboratory methods for biodosimetry

  1. Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies, (IAEA, PAHO, WHO, September 2011)
  2. Di Giorgio M, Barquinero JF, Vallerga MB, et al. Biological dosimetry intercomparison exercise: an evaluation of triage and routine mode results by robust methods. Radiat Res. 2011 May;175(5):638-49. Epub 2011 Feb 9. [PubMed Citation]
  3. Beinke C, Braselmann H, Meineke V. Establishment of an x-ray standard calibration curve by conventional dicentric analysis as prerequisite for accurate radiation dose assessment. Health Phys. 2010 Feb;98(2):261-8.
  4. Wilkins RC, Romm H, Kao TC, Awa AA, Yoshida MA, Livingston GK, Jenkins MS, Oestreicher U, Pellmar TC, Prasanna PG. Interlaboratory comparison of the dicentric chromosome assay for radiation biodosimetry in mass casualty events Radiat Res. 2008 May;169(5):551-60. [PubMed Citation]
  5. Bauchinger M, Blakely WF, Darroudi F, Edwards A, Fenech M, Hayata I, Koteles GJ, Lindholm C, Lloyd D, Lucas J, Prasanna PGS, Roy L, Sorokine-Durm I, Turai I, Voisin P: Cytogenetic Analysis for Radiation Dose Assessment: A Manual. (PDF - 859 KB) IAEA Technical Report Series No. 405, pp.127, Vienna, 2001
  6. ISO 19238:2004. Radiation protection - performance criteria for service laboratories performing biological dosimetry by cytogenetics. International Organization for Standardization (ISO).
  7. Voisin P, Barquinero F, Blakely B, Lindholm C, Lloyd D, Luccioni C, Miller S, Palitti F, Prasanna PG, Stephan G, Thierens H, Turai I, Wilkinson D, Wojcik A. Towards a standardization of biological dosimetry by cytogenetics. Cell Mol Biol (Noisy-le-grand). 2002 Jul;48(5):501-4.

Overview and limitations of cytogenetics as a biodosimetry tool

  1. Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies, (IAEA, PAHO, WHO, September 2011)
  2. Vinnikov VA, Ainsbury EA, Maznyk NA, Lloyd DC, Rothkamm K. Limitations associated with analysis of cytogenetic data for biological dosimetry. Radiat Res. 2010 Oct;174(4):403-14. [PubMed Citation]
  3. Leonard A, Rueff J, Gerber GB, Leonard ED. Usefulness and limits of biological dosimetry based on cytogenetic methods. Radiat Prot Dosimetry. 2005;115(1-4):448-54. [PubMed Citation]
  4. Lloyd DC, Edwards AA, Moquet JE, Guerrero-Carbajal YC. The role of cytogenetics in early triage of radiation casualties. Appl Radiat Isot 2000 May;52(5):1107-12. [PubMed Citation]
  5. Bender MA, Awa AA, Brooks AL, Evans HJ, Groer PG, Littlefield LG, Pereira C, Preston RJ, Wachholz BW. Current status of cytogenetic procedures to detect and quantify previous exposures to radiation. Mutat Res. 1988 Sep;196(2):103-59. [PubMed Citation]
  6. Brooks AL, Khan MA, Jostes RF, Cross FT. Metaphase chromosome aberrations as markers of radiation exposure and dose. J Toxicol Environ Health. 1993 Oct-Nov;40(2-3):277-88. [PubMed Citation]
  7. Bender MA, Gooch PC. Persistent chromosome aberrations in irradiated human subjects. Radiat Res. 1962 Jan;16:44-53. [PubMed Citation]

Using dosimetry to assess dose after partial-body irradiation

  1. Prasanna PG, Blakely WF, Bertho JM, Chute JP, Cohen EP, Goans RE, Grace MB, Lillis-Hearne PK, Lloyd DC, Lutgens LC, Meineke V, Ossetrova NI, Romanyukha A, Saba JD, Weisdorf DJ, Wojcik A, Yukihara EG, Pellmar TC. Synopsis of partial-body radiation diagnostic biomarkers and medical management of radiation injury workshop. Radiat Res. 2010 Feb;173(2):245-53. [PubMed Citation]
  2. Ainsbury EA, Livingston GK, Abbott MG, Moquet JE, Hone PA, Jenkins MS, Christensen DM, Lloyd DC, Rothkamm K. Interlaboratory variation in scoring dicentric chromosomes in a case of partial-body x-ray exposure: implications for biodosimetry networking and cytogenetic "triage mode" scoring. Radiat Res. 2009 Dec;172(6):746-52. [PubMed Citation]
  3. Prasanna PG, Moroni M, Pellmar TC. Triage dose assessment for partial-body exposure: dicentric analysis. Health Phys. 2010 Feb;98(2):244-51. [PubMed Citation]

Using biodosimetry after protracted exposure or delayed analysis

  1. Bertho JM, Roy L. A rapid multiparametric method for victim triage in cases of accidental protracted irradiation or delayed analysis. Br J Radiol. 2009 Aug;82(981):764-70. [PubMed Citation]

Increasing laboratory capacity for biodosimetry testing for emergency responses

  1. Chauhan V, Wilkins RC. Report of the workshop on biological dosimetry: increasing capacity for emergency response. Radiat Prot Dosimetry. 2010 Nov;142(1):2-7. [PubMed Citation]
  2. Wojcik A, Lloyd D, Romm H, Roy L. Biological dosimetry for triage of casualties in a large-scale radiological emergency: capacity of the EU member states. Radiat Prot Dosimetry. 2010 Mar;138(4):397-401. [PubMed Citation]
  3. Christie DH, Chu MC, Carr Z. Global networking for biodosimetry laboratory capacity surge in radiation emergencies. Health Phys. 2010; 98(2):168-171. [PubMed Citation]
  4. Leonard A, Rueff J, Gerber GB, Leonard ED. Usefulness and limits of biological dosimetry based on cytogenetic methods. Radiat Prot Dosimetry. 2005;115(1-4):448-54. [PubMed Citation]

Other biodosimetry techniques for dose estimation

  1. High dose radiation effects and tissue injury, Report of the Independent Advisory Group on Ionising Radiation, Table 4.4, page 41 (Health Protections Agency, UK)
  2. Bertho JM, Roy L, Souidi M, Benderitter M, Gueguen Y, Lataillade JJ, Prat M, Fagot T, De Revel T, Gourmelon P. New biological indicators to evaluate and monitor radiation-induced damage: an accident case report. Radiat Res. 2008 May;169(5):543-50. [PubMed Citation]
  3. Blakely WF, Ossetrova NI, Whitnall MH, Sandgren DJ, Krivokrysenko VI, Shakhov A, Feinstein E. Multiple parameter radiation injury assessment using a nonhuman primate radiation model-biodosimetry applications. Health Phys. 2010 Feb;98(2):153-9. [PubMed Citation]
  4. Graessle DH, Fliedner TM. Computer-assisted severity of effect assessment of hematopoietic cell renewal after radiation exposure based on mathematical models. Health Phys. 2010 Feb;98(2):282-9. [PubMed Citation]
  5. Ainsbury EA, Lloyd DC. Dose estimation software for radiation biodosimetry. Health Phys. 2010 Feb;98(2):290-5. [PubMed Citation]
  6. Alexander GA, et al., BiodosEPR-2006 Meeting: Acute dosimetry consensus committee recommendations on biodosimetry applications in events involving uses of radiation by terrorists and radiation accidents. (PDF - 518 KB) Radiat Meas. 2007 July;42(6-7):972-96.
  7. Dainiak N, Berger P, Albanese J. Relevance and feasibility of multi-parameter assessment for management of mass casualties from a radiological event. Exp Hematol. 2007 Apr;35(4 Suppl 1):17-23. [PubMed Citation]
  8. Albanese J, Martens K, Karkanitsa LV, Dainiak N. Multivariate analysis of low-dose radiation-associated changes in cytokine gene expression profiles using microarray technology. Exp Hematol. 2007 Apr;35(4 Suppl 1):47-54. [PubMed Citation]
  9. Chao NJ. Accidental or intentional exposure to ionizing radiation: biodosimetry and treatment options. Exp Hematol. 2007 Apr;35(4 Suppl 1):24-7. [PubMed Citation]
  10. Blakely WF, Salter CA, Prasanna PGS. Early-response biological dosimetry-Recommended countermeasure enhancements for mass-casualty radiological incidents and terrorism. Health Phy 2005 89(5):494-504 [PubMed Citation]
  11. Prasanna PGS, Blakely WF. Premature chromosome condensation in human resting peripheral blood lymphocytes for chromosome aberration analysis using specific whole-chromosome DNA hybridization probes. 2005 Methods Mol boil 291:49-57 [PubMed Citation]
  12. Prasanna PG, Escalada ND, Blakely WF. Induction of premature chromosome condensation by a phosphatase inhibitor and a protein kinase in unstimulated human peripheral blood lymphocytes: a simple and rapid technique to study chromosome aberrations using specific whole-chromosome DNA hybridization probes for biological dosimetry. Mutat Res 2000 Mar 23;466(2):131-41. [PubMed Citation]

Classical books and manuscripts about biodosimetry

  1. Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies, (IAEA, PAHO, WHO, September 2011)
  2. Lloyd DC. Chromosomal analysis to assess radiation dose. Stem Cells. 197; 15(Suppl 2):195-201
  3. Goans RE, Holloway EC, Berger ME, Ricks RC. Early dose assessment in criticality accidents. Health Phys. 2001;81:446-9. [PubMed Citation]
  4. Goans RE (2001) Clinical care of the radiation-accident patient: patient presentation, assessment, and initial diagnosis. In: Ricks RC, Berger ME, Ohara FM, Jr. (eds) The Medical Basis for Radiation-Accident Preparedness, The Clinical Care of Victims, Proceedings of the 4th International Conference REAC/TS Conference on The Medical Basis of Radiation Accident Preparedness, Orlando, FL, March 2001. Boca Raton, FL: The Parthenon Publishing, pp 11-22.
  5. Guskova AK, Baranov AE, Gusev IA. Acute radiation sickness: underlying principles and assessment. In: Gusev AE, Guskova AK, Mettler FA Jr, eds. Medical management of radiation accidents. Washington, DC: CRC Press; 2001: pages 33-51.
  6. MacVittie TJ, Weiss JF, Browne D (eds) (1996) Advances in the Treatment of Radiation Injuries, Vol. 94. Advances in the Biosciences. Oxford: Pergamon.
  7. Baranov AE, Densow D, Fliedner TM, Kindler H (1994) Clinical Pre Computer Performa for the International Computer Database for Radiation Exposure Case Histories. Berlin, Heidelberg, New York: Springer-Verlag.
  8. Dons RF, Cerveny TJ (1989) Triage and treatment of radiation-injured mass casualties. In: Walker RI, Cerveny TJ (eds) Medical Consequences of Nuclear Warfare, Part I, Vol. 2. In: Zajtchuk R, Jenkins DP, Bellamy RF, Ingram VM (eds) Textbook of Military Medicine. Falls Church, VA: Office of the Surgeon General, Department of the Army, United States of America, pp 37-54.
  9. Gusev IA, Guskova AK, Mettler FA Jr, eds.: Medical Management of Radiation Accidents, 2nd ed. Boca Raton, Fl: CRC Press, 2001.
  10. Jarrett, DG (1999) Medical Management of Radiological Casualties, First Edition (Special Publication 99-2; PDF available www.afrri.usuhs.mil ). Bethesda, MD: Armed Forces Radiobiology Research Institute.
  11. Waselenko JK, MacVittie TJ, Blakely WF, Pesik N, Wiley AL, Dickerson WE, Tsu H, Confer DL, Coleman CN, Seed T, Lowry P, Armitage JO, Dainiak N. Strategic National Stockpile Radiation Working Group. Medical management of the acute radiation syndrome: recommendations of the Strategic National Stockpile Radiation Working Group. Ann Intern Med. 2004 Jun 15;140(12):1037-51. [PubMed Citation]
  12. Mettler FA Jr, Upton AC: Medical Effects of Ionizing Radiation, 3rd ed. Philadelphia, Pa: Saunders Elsevier, 2008.

Correlation of radiation dose with signs and symptoms

  1. Fliedner TM, Friesecke I, Beyrer K. Medical Management of Radiation Accidents: Manual on the Acute Radiation Syndrome. (METREPOL) (PDF - 970 KB) (originally published by Oxford: British Institute of Radiology; 2001) Compendium to the main METREPOL document (PDF - 580 KB)
  2. Anno GH, Baum SJ, Withers HR, Young RW. Symptomatology of acute radiation effects in humans after exposure to doses of 0.5-30 Gy. Health Phys. 1989 Jun;56(6):821-38. [PubMed Citation]
  3. See also: REMM Time/Dose Effects in Acute Radiation Syndrome - Acute Clinical Effects of Single-Dose Exposure of Whole-Body Irradiation

Biodosimetry techniques in radiation epidemiology

  1. Simon SL, Bouville A, Kleinerman R. Current use and future needs of biodosimetry in studies of long-term health risk following radiation exposure. Health Phys 2010 Feb; 98(2): 109-17. [PubMed Citation]
  2. Radiation Dosimetry Monograph: "Applications of Dosimetry in Radiation Epidemiology" (Radiation Research, July 2006, Volume 166, Number 1. Special Supplement) (HHS/National Cancer Institute/Division of Cancer Epidemiology and Genetics)

The AFRRI BAT Computer Program

  1. See list of published references
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